Sampling Fish

Recently for work I got to help out in the field taking samples to quantify environmental contamination. Some of the samples we were taking were fish tissue to measure the levels of polychlorinated biphenyls (PCB) in them. The fish live in a river that was contaminated decades ago. The sampling results will be used for fish advisories and also to determine a clean up plan.

Sampling fish starts with the really fun part, which is cruising on a small electrofishing boat. Electroshocking the fish allows you to catch them alive and throw back any fish we didn’t want. We had target fish we were trying to catch to sample, and those were the only ones we kept, and we only the number of target fish we needed. The electroshock sort of stuns the fish but doesn’t kill them. The electrofishing boat has two long poles with anode wires hanging off of them protruding from the front of the boat. There were more wires hanging from the bow of the boat, and those are the cathodes. The electricity flows from the anodes to the cathodes. We stood at the front of the boat in rubber soled boots with nets extended waiting to catch any fish stunned by the electroshocking. Netting electroshocked fish is not actually as easy as it sounds. Some of the fish are more stunned than others, so some fish seem slightly confused but then swim away. Also, some were stunned but at a depth too low or cloudy for us to catch or see. According to the boat’s captain, the water had really low conductivity, which was making it difficult. Since we had target fish we were trying to catch, I, naturally, kept catching fish we didn’t want. I threw a lot of fish back. Still, a day on a boat catching or not catching fish was a wonderful change from the cubical I normally work in. Also, I learned that you really need polarized sunglasses when out on the water.

View from the boat with the anodes out in front. Not a bad office.

View from the boat with the anodes out in front and nets at the ready. Not a bad office.

Front of boat, cathode wires hang along bow

Front of boat, cathode wires hang along bow

Anode hangs in front of the boat

Anode hangs in front of the boat

Caught fish in boat's holding tank

Caught fish in boat’s holding tank

Once we got the fish to shore, the biologist took over. The fish were weighed and their length measured. He took a a sample of their scales from a standard location, and those scales were going to be used by a laboratory to determine their age. Evidently scales can be used to age fish in the same manner tree rings age trees. WARNING: If you are uncomfortable looking at the insides of fish, do not read any further. You should probably not eat fish also, if you can’t look at an uncooked one.

Scrapping scales off fish. Scales are used to age fish.

Scrapping scales off fish. Scales are used to age fish.

The rest of the scales were then scraped off. The fish were then cut. Only the fillets were used for sampling. The part of the fish used for sampling can differ depending on what the exposure pathway being examined is. We took two different parts: the filet, which represents what a human would normally eat, and also the fillet with rib meat. The rib meat is normally not eaten, but it would have more PCBs in it, so using it in the sample would represent a worse case scenario for a human consuming fish.

Cutting fish to take the samples

Cutting fish to take the samples

Fish samples ready for lab. Left side fillet also has rib meat. Right side fillet does not.

Fish samples ready for lab. Left side fillet also has rib meat. Right side fillet does not.

Fish post fillet and rib sample

Fish post fillet and rib sample

Fish post fillet sample

Fish post fillet sample

I also learned a bit of fish anatomy during the sampling. The biologist was also sexing the fish.

Female brown bullhead fish with orange egg sack

Female brown bullhead fish with orange egg sack

Male fish

Male fish with testes/seminiferous tubules indicated

We weren’t necropsying the fish, but we still got a look inside, including sometimes as to what it had eaten recently.

Fish gastro intestine tract. Eaten food is in stomach.

Fish gastro intestine tract. Eaten food is in stomach.

We sampled quite a few fish, but it was for science and to benefit the community.

Pile of fish that have had samples taken from them

Pile of fish that have had samples taken from them

Rebecca Kamen: Fundamental Forces

Currently on display at the National Academy of Sciences is Fundamental Forces by Rebecca Kamen. Fundamental Forces is an exhibition of paintings and sculptures inspired  the process of scientific discovery. The title Fundamental Forces refers to fundamental forces in physics: gravity, electromagnetism, and strong and weak nuclear interactions, and in my opinion, the exhibit really does provide a lovely representation of those fundamental forces. I loved the wire sculptures in particular as they were visually interesting  and also looked like things I had studied in chemistry and physics class. Matter Informing Space reminds me of the Bohr model of the atom. The Doppler Effect is an interesting visualization of the Doppler effect, but it also reminds me of a vortex and the Coriolis effect. The sculptures also play with the light in wonderful ways. The shadows created by the wire sculptures and also Portal are incredibly interesting. If you are in the DC area, the exhibit is open until July 6, and it is free to see, so go.

Doppler Effect

Doppler Effect

Doppler Effect

Doppler Effect

Wave Ride for Albert

Wave Ride for Albert

Wave Ride for Albert

Wave Ride for Albert

Cosmos: For Carl

Cosmos: For Carl

Cosmos: For Carl

Cosmos: For Carl

Matter Informing Space

Matter Informing Space

Matter Informing Space

Matter Informing Space

Matter Informing Space

Matter Informing Space

Portal

Portal

Portal

Portal

Portal

Portal

Sky DIary

Sky Diary

Magic Circle of Circles

Magic Circle of Circles

Matrix 1

Matrix 1

It’s Not Melting, It’s Dissolving

Time for another post where I get on my science grammar soapbox. Have you ever seen the movie The Wizard of Oz? Everyone has seen that movie, right? Recall the scene where Dorothy throws a bucket of water on the Witch, and the Witch dissolves into a puddle while screaming “I’m melting, I’m melting”? First, don’t ever watch that movie with me. Why? Because every time I see that scene, I scream “you’re not melting, you’re dissolving, get it right.” Ok, it is a movie, a movie that takes place where monkeys fly, there are witches, lions walk and talk like humans, and scarecrows come to life. No, I shouldn’t be looking for realistic science in it. However it drives me crazy that they can’t even get the simple difference between dissolution and melting correct.

In the exact same incorrect way, there is a saying that some people say when their children, dog, whatever, is hesitate to go outside in the rain. “You are not made of sugar, you won’t melt.” There are actually several things wrong with that statement. Sugar, as in table sugar, which is specifically sucrose (as opposed to all the other sugars that exist), does not actual melt at all. At 186°C (367°F), it decomposes to caramel. So even if that saying meant decomposes, if the temperature outside is high enough for sugar to decompose, you have much bigger problems then possibly getting wet. You would die of heat. However, if you were made of sugar, and you went out into the rain, you would not have to worry about melting, you would have to worry about dissolving.

Melting is a physical process where solid turns into a liquid due to heat applied to it. Stick ice into a glass at room temperature. Wait a while. You now have water in the glass. The ice melted into water. But you didn’t apply heat, you might argue. The melting point of ice, the temperature at which solid water, i.e. ice, becomes liquid water is 0°C (32°F). So by simply having ice at room temperature (around 22 °C (72 °F)), heat has been applied to it. The temperature is higher than what the ice needs to stay a solid. Similarly put solid chocolate in a pot and heat slowly to 30°C (86°F). You have liquid chocolate. It has melted. Now don’t waste that chocolate, go eat it with strawberries or cake. [Excuse me for a moment. . .]

Now take that glass of water you made by melting ice at room temperature, and pour just a little salt into it. The salt has dissolved into the water. The water, which is the solvent, has dissolved the salt, the solute, into a solution. When Dorothy throws water on the Witch, the Witch is the solute, the water is the solvent again, and now you have a witch solution in water. Based on the film, witch dissolves quite readily. [It would not matter if she threw boiling water on the Witch, it would still be dissolution because the water is mixing with the witch. The water was quite clearly not boiling anyway.] Other liquids can act as solvents to dissolve solutes, but water is the most common in everyday life. Wiping acetone on nails painted with nail polish removes the polish because acetone, a solvent, dissolves the hardened nail polish, the solute, into a solution. [It is a temporary solution in the sense that acetone readily evaporates, but it forms a solution with the polish long enough to transfer the polish to a cotton ball. The acetone then evaporates leaving behind the polish on the cotton.] An important distinction between melting and dissolving is that melting only involves one substance, water, chocolate, wax, etc. Dissolution involves two substances, water and salt, water and sugar, acetone and nail polish, etc. Dissolution can also involve applied heat, but it isn’t required. There is a much longer explanation for that, and it relates to the solute and solvent and numerous other factors.

To review, melting is one substance changing from a solid to a liquid, and one, and only substance is involved. It is a phase change that must involve a temperature (or pressure) change. Dissolution is one substance becoming part of a solution with a liquid, and two substances are involved. It is two substances becoming one, and temperature change is not necessary for it to happen.

I honestly don’t understand why some people don’t understand the difference. However ignorance of this appears to be wide spread. Evidently the U.S. Special Inspector General for Afghanistan Reconstruction does not know the difference. That Inspector General recently released a report concerning a half-million-dollar U.S.-built police training center in Afghanistan that was so badly constructed that it is literally “melting.” Nope, it is not. It is literally dissolving. If the center had been made of wax, then maybe it might melt. Based on the wording in this article and the accompanying photos, the building is quite clearly dissolving. That is still incredibly appalling construction. As an engineer, I would really like to see the design plans. However, if the Inspector General does not even know the difference between melting and dissolving, then perhaps the Inspector General would do well to have someone on staff who does. It would make for better and more accurate reports.

WMATA L’Enfant Plaza Fire

Today there was a fire in a WMATA subway tunnel near L’Enfant. The National Transportation Safety Board (NTSB) is now investigating, and I have confidence that they will do a thorough investigation. I have some questions about actions taken right after the smoke was reported that no one, or at least no one in the media I have seen, has asked. The station filled with smoke, and they evacuated it. WMATA stopped running green and yellow trains through L’Enfant. However they kept running blue, orange, and silver trains through, but these trains did not stop at the station, as they normally would. For those not familiar with L’Enfant Plaza Metro Station, orange, blue, and silver lines share the same track on the lower level, and yellow and green share the same track on the upper level. My question is, were they sure it was safe to keep sending the orange, blue, and silver trains through? I am not asking from the standpoint of the fire, because presumably, they traced the location of the smoke enough to know it was not in the lower tunnel. By safe, I mean because of the potential inhalation of smoke in the lower level tunnels. If the station filled with enough smoke that it needed to be evacuated, then how were they sure that smoke would not enter the trains running through it?

A couple of quick points:

  • Just because air smells bad doesn’t necessarily mean it is toxic or hazardous, but conversely, just because air smells fine doesn’t mean it is safe.
  • Particulate matter in air and/or smoke is in general not something you really want to breath, but there are different levels of toxicity associated with it. That is, some particulate matter is not more than just an irritant. However, the effect particulate matter has on a person is also affected by that person’s health. People with respiratory issues are more susceptible to any effects.
  • Exposure to hazardous or toxic materials can cause effects on different time scales. People who were trapped on the WMATA train in the tunnel, would have acute (short-term) effects from breathing the smoke, such as coughing and having trouble breathing. However, they were probably also exposed to chemicals whose effect is not immediate, such as carcinogens.

The questions I have, that I have not heard anyone ask include:

  • What is the air exchange rate between the subway trains and the surrounding air? Can the ventilation be turned off manually, so that there was no air exchange between the train and the surrounding air while the trains were near L’Enfant?
  • Were there any measurements taken of the air in L’Enfant, particularly on the lower level where the orange, blue, and silver trains were still running through? If so, what were the measurements of? Just measuring particulate matter will not indicate almost nothing about organic compounds or other chemicals in the air.
  • How far did the smoke spread?
  • Assuming air measurements were taken, did anyone calculate the amount of contaminants that people in the trains would be exposed to while running through the station based on time and air exchange rate?

My educated guess is that no air measurements were taken. There are probably some sensors in place to measure smoke, but depending on how that measurement is taken, it will tell you information about the particulate matter and that is it. I seriously doubt there was initially any sensors that measured organic compounds or any other type of compounds in the air. I have my doubts that any portable system was put in place during the response. The priority would have been evacuating people (as it should have been). It is possible that WMATA had some qualitative data that there was not much smoke on the lower level. That is, someone may have looked at a video screen and decided the air didn’t look bad. However, unless they had actual quantitative data of what was in the air, then visual assessment of air is a really bad way to make assessments on the quality of the air.

The early statements by WMATA and all other sources, like the fire departments involved, was that they did not know the source of the fire, location or cause. Thus they could not have possibly known what was burning and what would be in the air. For example, if wood is burning, you can expect certain chemicals in the air. If rubber is burning, you can expect different chemicals in the air. WMATA probably decided that the air on the lower level didn’t look that bad, and the trains would go through the station quickly enough that very little exposure would occur. They very well may be right, but with no data and no statements about any calculations, they have no way to prove that. Also, did they inform their passengers of this? If I was on a train, and I knew that the train was going to go through, but not stop, at a station that was filling with smoke, I would get off the train. I don’t feel the need to expose myself unnecessarily to hazardous substances, even if in small amounts. I do not like standing near people who are smoking. The second hand smoke may only minimally increase my risk of disease, but I still don’t see the need for that tiny increase. Thus, was WMATA considering passengers’ exposures at all? Furthermore, did they communicate the possibility of exposure to their passengers on the orange/blue/silver lines to allow their passengers to make their own educated decision about staying on the train? My guess is the answer to both those questions is no, and that is another thing to which WMATA should be made to respond.

Collapse

One of the exhibits, we visited during Cultural Programs of the Natural Academy of Sciences’s #NAS_sciart was Brandon Ballengée: Collapse. Collapse is a huge pyramid of specimen jars that contain species from the Gulf of Mexico that are in decline due to the 2010 BP Deepwater Horizon oil spill. It was interesting to be able to see numerous species up close, even if preserved, that I normally would never see. However, it was sad to think that all these species are in decline or at least affected by the Deepwater Horizon oil spill.

Collapse

Collapse

Collapse

Collapse

Crabs

Crabs

Flouder

Flouder

Giant sea roach

Giant sea roach

Octopus

Octopus

Shells

Shells

Fish

Fish

Turtle

Turtle

Void

I went to an Instameet at the National Academy of Sciences today, where we toured two art exhibits and also the building. One of the exhibits we toured was Imagining Deep Time, and one of the pieces of art in that exhibit is Chul Hyun Ahn’s Void. I have seen this exhibit before, and no matter how many times I see Void, it still freaks me out. It just does. The piece is made of cast acrylic, LED lights, hardware, and mirrors, and while it physically has a size of 90 x 71 1⁄2 x 12 1⁄4 inches, it looks like a tunnel that goes on forever. The photos I have posted below simply do not do it justice. I am very impressed by how the artist was able to create the vision effect, but when I stand in front of it, I keep waiting for Dr. Who, Spock, or some other science fiction character to come walking through the tunnel. I guess it kind of scares me. Yes, I am a wimp. A piece of art that is just lights, mirrors, and acrylic scares me.

Side note: The photo of Void with JD Talasek, the curator of Imagining Deep Time, in front of it, is the only photo posted below that I took with my iPhone. The rest of the photos I took with my Canon 6D. I note this because I find it interesting how the two cameras captured the light differently. The only manipulation I performed on my photos was cropping and some straightening.

A view of the whole of Void

A view of the whole of Void

JD Talasek, the curator of Imagining Deep Time, stands in front of Void

JD Talasek, the curator of Imagining Deep Time, stands in front of Void

The "floor" formed by Void

The “floor” formed by Void

Edge of Void, where the visual effect hits reality

Edge of Void, where the visual effect hits reality

A selfie in Void

A selfie in Void

It’s Not Individualism or Bad Fashion, It’s Sexism

I once hypothesized that male heterosexual scientists and engineers single-handily keep the Hawaiian shirt industry in business. Don’t ask me why, but as a group, they love those shirts. I make jokes about their lack of fashion and just plain dressing ability. I tease because I love. I love their individualism, and I love how they don’t know or care about fashion. I may be a female heterosexual scientist and engineer, but I am one of them when it comes to dress. The last time I remember being fashionable was when I was in fifth grade. I don’t understand or like many fashions. I have my own style, and I like to look nice, but I consider my ability to wear jeans to work and not even own a suit, a serious perk of my career (and employer).

And then there is this.

This is Rosetta Project Scientist Matt Taylor of the European Space Agency (ESA) in a shirt covered in scantily clad women in in sexually suggestive poses. That is the shirt he chose to wear on a day when ESA did the amazing feat of landing a probe on a comet. This is the shirt he chose to wear on a day when he would be interviewed by the media and featured on live webcasts of the events. Not only did he not see a problem with this shirt, but evidently no one else at ESA did either. This. Is. Not. Acceptable. This is not appropriate. This is offensive. This shirt should not exist period, but it most certainly should not exist in the workplace. This is not about how ugly the shirt is. This is not about how unprofessional a shirt like that is. This is not about Dr. Taylor being an individual and expressing his style. This is about a shirt that objectifies women. This is about a shirt that is sexual harassment without Dr. Taylor even opening his mouth or making any type of gesture or doing absolutely anything other than wearing it. This is about a complete and utter lack of respect of women on the part of Dr. Taylor and evidently everybody at ESA who works with him and would have been in a position to say something. This about no one over there seeming to care about whether or not women feel comfortable working there when someone can wear a shirt like that. This about telling women it doesn’t matter your intelligence, skills, education, or ideas, you are but sex objects. The STEM fields continue to have a problem with sexism and gender inequality. My alma mater, a technical college, still only has about a 25% female student body. Wearing shirts like that to workplace will not help. It will not tell women that they are welcome. I quite frankly don’t care if Dr. Taylor is actually a really nice guy who is actually very supportive of women in STEM. His shirt says otherwise. He and ESA owe all of us an apology. That shirt overshadowed what should have been the main headline that ESA did the absolutely spectacular task of landing a probe on a comet. That shirt and the attitude it expressed ruined it for me in fact.

Finally, I would like to give mad props to Dr. Paul Coxon for his absolutely awesome idea, that if you want to wear a shirt with women on it, wear one with these women on it.


These would be some of the women of the Indian Space Research Organisation (ISRO) celebrating after ISRO’s Mars Orbiter Spacecraft successfully entered the Mars orbit. And they are awesome.

Do not mess with a toxicologist

I was attending a lecture today on toxicology, and of course the subject people purposely poisoning other people came up. The lecture relayed the story of a female chemist who had a romantic relationship with a male chemist who worked in the same lab. The male then broke off the relationship and starting dating another female chemist who also worked in that same lab. The first female chemist evidently pretended like she was ok with everything and continued to make tea for everyone afterwards. However she started adding acrylamide to the male’s tea. It sounded like the poisoning was discovered fairly quickly. He then relayed another story of similar circumstances when a male broke off a relationship with another woman, but they continued to work together. She used thallium to poison his tea. She however was a toxicologist and made detailed notes of the effects on the male. She then adjusted the dosage accordingly. My lecturer did not relay information as to when she was caught. Summary of the story is don’t piss off a toxicologist, and if you do, don’t be stupid enough to let the person make your tea.

DISCLAIMER: This is in no way meant to encourage people to poison people. Don’t do that. Seriously, don’t.

Old Medical Journal Memos

I have a confession. Sometimes I am not productive as I should be because my curiosity gets the better of me. I need to look something up, and in the course of researching it, I read some other tangental tidbit, which cause me to look something else up, and then down the rabbit hole I go. Case in point was today. I have a project at work that involves a database of environmental contaminants. The short version is, these contaminants are in the database under numerous synonyms, including synonyms that are insanely obscure and uncommon, or perhaps they were common a hundred years ago, but certainly not today. All the contaminants are in the database associated with their CAS Registry Numbers, so it is clear what the chemical is. [A CAS Registry Number is like a Social Security number for a chemical. It is specific to a chemical or a specific type of mixture of chemicals.] Many of these synonyms I have never heard of before. One synonym for nitrobenzene was oil of mirbane. I had to look this one up for pure curiosity reasons. After much searching, I have yet to find out the etymology of oil of mirbane or simply the word mirbane. [@vonOberst on Twitter suggested thusly “Mirer is a candle in French. Bane is “fatal”…maybe someone tried to make a candly from nitrobenze crystals?” For the non-chemists, nitrobenzene is among other things, explosive, so a candle made from it would definitely be a fatal candle, and I find this idea disturbing and amazing. If anyone knows the etymology I would seriously love to know from where this name came.]

Anyway, in the course of searching for the origins of oil of mirbane, I came across a British Medical Journal memoranda from Jan 27, 1912 (1(2665): 183) about someone who was accidentally poisoned with it. The article didn’t get me to a better understanding of that name, but it was an interesting article, and I became interested in these old memoranda and the way they were written, diagnosis, and treatment. So then I had to read a few more in this volume, and now reading old medical journal memoranda is going to become my new hobby. They are fascinating. However, I need to share part of my favorite one from that volume, “A supposed case of heat-stroke: remarkable recovery.” This report is completely fascinating, and I would really love to know from what this patient was suffering. I have no medical training, but this does not sound like heat stroke as it has been described to me in first aid training. The case as described.

“A man, aged 53, was crossing a road during one of the hottest days of last summer, when he suddenly found himself on the ground, with a “horse’s hoof right on top of him”; this was his description of what happened. He picked himself up and ran across the road to his son, who was waiting for him on the pavement, and who brushed the dust off his clothes.He complained of no pain or discomfort of any kind, but his son took him into a chemist’s shop, where he was given a dose of sal-volatile. He then went home by train (a distance of eight miles), sat down and made a good tea, feeling quite well all the time. Towards the end of this meal he became a “little queer,” went upstairs and felt very ill indeed, and remembered nothing afterwards. This attack came on about five hours after he had fallen down in the road.”

Read the whole article. It is not long and is truly fascinating. Included is the information that the doctor examined him and found no injury, which kind of calls into the question the idea of a horse hoof on him. I am not sure what I love the most about this article, but the pure Britishness of it is definitely part of it. I adore the details that the son helped brush dust off the man, which makes me wonder what the son was doing when the man fell down in the road or whatever he did. Also, of course the man went home and made a good tea. The man recovered after his attack over the three days, then he got worse. Part of the treatment that was given to him when he fell unconscious was withdrawal of cerebrospinal fluid. It never states why. The patient got better after that, then got worse, then they removed more cerebrospinal fluid, and he got better again. Evidently his “heat stroke” was cured by removing cerebrospinal fluid. Is this still something done for heat stroke? How exactly does removing cerebrospinal fluid help heat stroke or anything? I actually would like to know why this was done, or what the theory was back then (or now?!).

Thus now, I reading these old medical memoranda is going to be my new thing during my free time. The article before the heat stroke memoranda describes two children suffering from tetanus infections who were treated unsuccessfully with magnesium sulfate injected into their cerebrospinal fluid. One of the children was also given strychnine. This makes me all the more thankful for tetanus vaccines, even if they do hurt.

Twitter, Scientists, and Arbitrary Lists

Fairly often some website produces a list of people you should follow on Twitter. Yesterday it was Science with their The top 50 science stars of Twitter. This list, like so many before, is arbitrary, lacks diversity, and is based on, in my opinion, stupid metrics. Many people on Twitter have noted that this list is overwhelmingly white and male. They based the star status on follower count and a completely ridiculous metric called the Kardashian Index,” or K-index, which is about as ridiculous as the people for which it is named. The list also lacks diversity from a field of study standpoint. Also, some people have noted that other “star” Twitter scientists were left off, which according to the article’s author was because they restricted the list to Ph.D.s. I think that is a stupid restriction, and I am a Ph.D. Furthermore, someone noted that one of the accounts on the list is a bot, and another one are simply tweets by the person’s PR person. 

I follow a few people on the list, so obviously I think some of them worth following. However, if you are trying to be more active on Twitter and interact with people, most (but not all) of these people are not the people to follow. The more followers you have, the more difficult it is to interact with them, assuming you are even trying. Don’t get me wrong, some of the people on this and other lists do tweet great information. However, if your goal on Twitter is to network, make friends, learn things, and sometimes get help or advice, then “stars” are not to the people to follow. I have made friends on Twitter, including friends I have later met in person. I have also networked and gotten great advice on work and personal projects. I see tweets on an almost daily basis of scientists helping each other out via tweets. Someone will tweet out asking for advice on some lab protocol or best manner to collect a certain type of sample, and others will reply with advice. Many people, including myself, tweet out a photo of something we are trying to identify. If I know people who know things in that field, I’ll tag them, and via crowdsourcing, we can normally identify the life form or object. That sort of fun learning experience is through interactions with us non Twitter stars.

If you want to use Twitter for things like that, you need to seek out people in your field or fields you are interested in, or just people who tweet out interesting things. Ignore the number of followers they have, and look at what they tweet. The less followers they have, the more likely they will follow you and interact with you. There are wonderful people with tons of followers that are worth following on Twitter, and some of them do a good job of interacting, and there are some worth following even if they don’t interact. I just mean that you can get a lot more out of Twitter if you interact with people. That leads to the obvious question, how do you find these people? Look for Twitter lists such Women Tweet Science Too which was created to in reaction to the lack of women on the above mentioned Nature list. Many people have already created tons of great public lists like this for people in various fields. Follow people on these, and then once you find people you really like on Twitter, see who they follow and with whom they interact.

Furthermore, if you want my personal opinion on how to get people to follow you, which you can take or leave, then see below.

1. Tweet. That may seem obvious, but it you don’t tweet, people are not going to follow you. Tweet links to articles you find interesting. Tweet things you find funny. Tweet about what you are working on, even if you think it is uninteresting or no one will understand what you are doing. Your fellow nerds and geeks will understand and be interested. Even if no one if following you, you have to get started somehow.

2. Have a avatar photo. Having one that represents something about you, even if it is not a photo of you. I rarely follow Twitter eggs.

3. Have a Twitter bio. When someone follows me, I look at their bio. Do they work in a field interesting to me? Do they say something funny? Do they have interests similar to me?

4. Interact with people. Even if a person doesn’t follow you, if they ask a question you can help with, reply to them. Give your input.